WETTABILITY AND DRUG DELIVERY OF FUNCTIONALLY GRADED NANO-MICRO POROUS TITANIUM SURFACE
윤귀덕, 박상원, 양홍소, 방몽숙, 박하옥, 임현필,
소속 상세정보
윤귀덕 ( Yun Kwi-Dug ) - 전남대학교 치의학전문대학원 치과보철학교실
박상원 ( Park Sang-Won ) - 전남대학교 치의학전문대학원 치과보철학교실
양홍소 ( Yang Hong-So ) - 전남대학교 치과대학 보철학교실
방몽숙 ( Vang Mong-Sook ) - 전남대학교 치과대학 치과보철학교실
박하옥 ( Park Ha-Ok ) - 전남대학교 치의학전문대학원 치과보철학교실
임현필 ( Lim Hyun-Pil ) - 전남대학교 치의학전문대학원 치과보철학교실
KMID : 0362420080460030307
Abstract
Statement of Problem: It is known that an anodic oxidation technique, one of the methods for the implant surface treatment, remarkably increased surface area, enhanced wettability and accelerated the initial bone healing.
Purpose: This study was performed to evaluate the wettability of anodized titanium surface which has a nanotubular structure, to assess osseointegration after the placement of implant with nano-size tubes on tibia of rats and to analyze quantitatively transferable rhBMP-2 on each surface.
Material and Methods: Four different kinds of surface-treated titanium discs (polished (machined surface) group, micro (blasting surface) group, nano (anodizedmachined surface) group, and nano-micro (anodized-blasting surface) group) were fabricated (n=10). Three different media were chosen to measure the surface contact angles; distilled water, plasma and rhBMP-2 solution. After a single drop (0.025 ㎖) of solution, the picture was taken with the image camera, and contact angle was measured by using image analysis system. For the test of osseointegration, 2 kinds of anodized surface (anodized-machined surface, anodized-blasting surface) implants having 2.0 ㎜ in diameter and 5.0 ㎜ in length inserted into the tibia of Wistar rats. After 3 weeks, tibia were harvested and the specimens were stained with hematoxylin and eosin for histological analysis. To test the possibility of drug delivery, after soaking sample groups in the concentration of 250 ng/㎖ of rhBMP-2 for 48 hours, the excess solution of rhBMP-2 were removed. After that, they were lyophilized for 24 hours, and then the rhBMP-2 on the surface of titanium was resolved for 72 hours in PBS. All the extracted solution was analyzed by ELISA. One-way analysis of variance (ANOVA) was performed on the data.
Results: The wettability is improved by anodic oxidation. The best wettability was shown on the nano-micro group, and it was followed by nano group, micro group, and polished group. In the histological findings, all implants showed good healing and the new bone formation were observed along the implant surface. After 3 days, nano-micro group delivered the most amount of rhBMP-2, followed by nano group, micro group, and polished group.
Conclusion: It indicated that anodic oxidation on blasting surface produce functionally graded nano-micro porous structure and enhance hydrophilicity of the surface and osseointegration. The findings suggest that the nano-micro porous structure could be a useful carrier of osteogenic molecules like rhBMP-2.
키워드
Wettability;Drug delivery;Nano-micro porous;Titanium surface
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